If I set up my iPod as a new iPod on iTunes will I lose all of the content on my iPod?

I've had my iPod touch for about a year but I have never actually synced it with iTunes (I just used the iTunes app)

and now I'm wanting to sync it with iTunes so I can put more music on it

 

I was wondering if I set up my iPod as a new iPod on iTunes will I lose all of the content on my iPod?

If so, is there any other way to do this?

 

I just want to know so that I don't set it up as a new iPod and then I lose all of my stuff

Thanks!!

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